Sensitivity of Terminalia catappa L. Toward Aeromonas hydrophila
(1) Universitas Riau
(2) Universitas Riau
(3) Universitas Riau
(*) Corresponding Author
Abstract
Terminalia catappa is commonly present in tropical area and its can be used for increasing pH in fish culture and it also be used as disinfectant. A research aims to understand the dosages of T. catappa leaf extract to hamper the growth of Aeromonas hydrophila has been conducted from February to July 2019. A diffusion agar KIRBY BAUER method was applied to understand the sensitivity of T. catappa toward the A. hydrophila. Treatments applied were P0 (Control, Oxytetracyclin (30 µg.Disk), P1 (100%), P2 (90%), P3 (80%), P4 (70%), P5 (60%), P6 (50%), P7 (40%), P8 (30%), P9 (20%), P10 (10%) P11 (9%), P12 (8%), P13 (7%), P14 (6%), P15 (5%), P16 (4%), P17 (3%), P18(2%), P19 (1%). Pure leaf extract was obtained from fresh leaf. The disk blank was wet with 50 L of leaf extract. A. hydrophila was cultured in TSA, TSB and GSP media, and it was grew up for 24 hours. Results shown that the pure leaf extract produce 13.9 mm diameter clear zone, while 2% of pure extract produce 6.5 mm diameter clear zone.
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DOI: http://dx.doi.org/10.31258/jpk.25.2.116-122
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